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Microbiology & Humanities Sciences

Chromatography and Mass Spectrometry Platform

Description: 

 

The chromatography and mass spectrometry technical platform is attached to the « Proteomics and functional analysis of proteins » team. The APHM staff works under the supervision of Pr Chabrière.

 

The platform team assists both hospital biologists and IHU researchers and develops expertise following two missions:

1. Clinical diagnosis: identification of bacteria and organisms (Biotyping by MALDI-TOF-MS) and antibiotics quantification (UHPLC-UV).

2. Method Development:

- Biotyping: identification of organisms and biological markers using MALDI-TOF-MS,

- Proteomics: identification of proteins in complex mixtures, relative proteomics (label free protein expression comparison), de novo peptide sequencing,

- Small chemicals and metabolites: identification and quantification of small molecules using liquid and gas chromatography (permanent gases, fatty acids, amino acids, pesticides, antibiotics, lipids, …)

 

Intranet website: access (only available in the laboratory)

 

Instruments: 

 

Biotyping:

- 8 MALDI-TOF-MS Microflex LT (Bruker 2010-2012)

- 1 MALDI-TOF-MS Autoflex Speed (Bruker 2016)

Proteomics:

- FreedomEVO 100 robot (Tecan 2013)

- nanoAcquity 2D-LC (Waters 2013)

- Synapt G2Si hybrid spectrometer (Waters 2013)

Small chemicals:

- UHPLC-UV/FLUO H-Class (Waters 2012)

- Headspace-GC/MS (Perkin Elmer 2013)

- GC-TCD (Perkin Elmer 2016)

- UHPLC-IMS-QTOF (iClass-Vion, Waters 2017)

 

Team supervisor:

 

- Pr Eric CHABRIERE (eric.chabriere@univ-amu.fr)

 

AP-HM staff:

 

- ARMSTRONG Nicholas (nicholas.armstrong@univ-amu.fr), Engineer: small chemicals and metabolites (GC/MS, LC/MS and LC-UV/FLUO),

- COUDERC Carine (carine.couderc@univ-amu.fr), Engineer: Biotyper research and routine assays (MALDI-TOF-MS)

- DECLOQUEMENT Philippe (philippe.decloquement@univ-amu.fr), Engineer: Proteomics (MALDI-TOF-MS and LC/MS)

- PICAULT Luc (luc.picault@ap-hm.fr), Engineer: Biotyper research and routine assays (MALDI-TOF-MS)

- RICHEZ/PHAM-VAN Magali (magali.richez@ap-hm.fr), Technician: small chemicals (GC/MS, LC/MS and LC-UV/FLUO),


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